Researchers used structured light and switchable fluorescent molecules to reduce the background light from the out-of-plane regions of microscope samples. This method allowed for the acquisition of ...
What is Structured Illumination Microscopy? Structured Illumination Microscopy (SIM) is a super-resolution fluorescence microscopy technique that allows for imaging beyond the diffraction limit of ...
SIMIP enables high-resolution images rich in both chemical and spatial information. A quantum cascade laser (QCL) excites molecular vibrations while a spatial light modulator (SLM) generates striped ...
Top: Random Illumination Microscopy (RIM) utilizes random speckle patterns to achieve super-resolution imaging of biological tissues, improving resolution by a factor of two compared to the optical ...
Researchers have shown that consumer-grade 3D printers and low-cost materials can be used to produce multi-element optical components that enable super-resolution imaging, with each lens costing less ...
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Triangle structured illumination microscopy developed for sustained live-cell super-resolution imaging
Structured illumination microscopy (SIM) is the most preferable system for live-cell super-resolution imaging. It enables the observation of intricate subcellular dynamics. However, conventional SIM ...
Even those who maintain that super-resolution microscopy is a powerful tool of biological discovery have admitted that it may have a bit of an image problem. For example, in a recent review, several ...
More information: Pengcheng Fu et al, Breaking the diffraction limit in molecular imaging by structured illumination mid-infrared photothermal microscopy, Advanced Photonics (2025). DOI: ...
This is especially true when studying intricate details beyond the optical resolution limit using structured illumination microscopy (SIM). SIM uses multiple images acquired under structured ...
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